Journal: Nucleic Acids Research

Article Title: Mitochondrial DNA replication is regulated by endoplasmic reticulum-mitochondrial contact sites, the mitochondrial calcium uniporter, and manganese

doi: 10.1093/nar/gkag233

Figure Lengend Snippet: Gene-silencing of Superoxide dismutase 2 inhibits mtDNA replication and is rescued by manganese. ( A ) si SOD2 or non-target (NT) silenced human fibroblasts were labelled with BrdU for 8 h and immunostained with anti-BrdU (green) and anti-TOMM20 (red). Scale bar = 50 µm. ( B ) Isolated protein from fibroblasts was immunoblotted for SOD2 after fractionation by SDS–PAGE, and quantified relative to VCL ( n = 4 independent experiments). ( C ) Quantification of the cytoplasmic BrdU foci per cell in 131 control (Ctrl) cells and 131 control cells supplemented with 50 µM MnCl 2 for 16 h, P = .89. 131 Ctrl versus 119 si SOD2 cells P = .03; and 119 si SOD2 cells versus 137 si SOD2 + Mn 2+ cells P = .03 ( n = 4 independent experiments). ( D ) EdU pulse-chase in primary human fibroblasts after transfection with siNT, si RTN4 , or si SOD2 . The EdU pulse was 26 h, and EdU incorporation in cytoplasmic foci was measured after chases of 0, 4, and 24 h. The chart represents the quantification of the EdU foci per cell in 26 non-target (NT) cells, 31 si RTN4 and 28 si SOD2 cells after a EdU pulse of 26 h (no chase); 46 NT cells, 57 si RTN4 , and 52 si SOD2 cells after a 4-h chase; and 41 NT, 36 si RTN4 , and 30 si SOD2 cells after a 24-h chase ( n = 3 independent experiments).

Article Snippet: All chemicals, unless otherwise stated, were purchased from Sigma–Aldrich with respective CAS number: thapsigargin (67526-95-8), tunicamycin (11089-65-9), 5-bromo-2′-deoxyuridine (BrdU) (59-14-3), 5-ethynyl-2′-deoxyuridine (EdU) (61135-33-9), EdU Click-it ® Thermo Fisher ( C10337 ), MCU-i4 (HY-138620, MedChemExpress), MnCl 2 (13446-34-9), cyclohexamide (66-81-9), actinonin (13434-13-4), HPG Click-it ® Thermo Fisher ( C10186 ).

Techniques: Isolation, Fractionation, SDS Page, Control, Pulse Chase, Transfection

Journal: Bioactive Materials

Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair

doi: 10.1016/j.bioactmat.2026.02.002

Figure Lengend Snippet: Multi-enzyme activity and ROS-responsive platform construction. (A) OXD-like enzyme activity at pH = 5.5. (B) OXD-like enzyme activity of MMBOx under different pH conditions. (C) POD-like enzyme activity at pH = 5.5. (D) POD-like enzyme activity of MMBOx under different pH conditions. (E) CAT-like enzyme activity at pH = 7.4. (F) CAT-like enzyme activity in five cycles for MMBOx. (G) K m and V max of MMBOx. (H) CAT-like enzyme activity of MMBOx under different pH conditions. (I) SOD-like enzyme activity under different concentration conditions at pH = 7.4. (J) SOD-like enzyme activity in five cycles for MMBOx. (K) Michaelis-Menten curves measured by EST-8 method for MMBOx. (L) DPPH scavenging curves at pH = 7.4. (M) Schematic diagrams of the mechanism of activation of enzyme activities by MMBOx in different environments. (N) Schematic illustration of GelMA-PBA synthesis and dual-network hydrogel formation. (O) Photographs of 3D-printed scaffolds with BOx and MMBOx incorporation (scale bars: 2 mm). (P) Storage modulus (G′) and loss modulus (G″) of different groups. (Q) Stress-strain curves of hydrogels. (R) Swelling kinetics of hydrogels. (S) H 2 O 2 scavenging efficiency of different groups.

Article Snippet: NaBiO 3 , NaOH, MgCl 2 , MnCl 2 ·H 2 O, MB, DPBF were from Macklin Biochemical Technology Co., Ltd (Shanghai, China).

Techniques: Activity Assay, Concentration Assay, Activation Assay